The primary goal of the present study was the development of highly user-friendly, charge-free and open source computer software to assess the quantitative aspect of liver fibrosis in a standardized and reproducible manner. Therefore, we used the model of bile duct ligation (BDL) to induct liver fibrosis in C57/BL6 mice. The experimental model has been well described and evaluated in rats and mice [9, 10] and has been widely used to study cholestatic liver injury [11, 12] and fibrogenesis [9, 13, 14]. We analysed time-related quantitative and semi-quantitative aspects of murine liver fibrosis and evaluated the different measurement techniques.
Quantitative Techniques Terry Lucey 6th Rar
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